Reaction Steps
A solution of tert-butyl 4-[N-[7-chloro-3-(2-dimethylaminoethyl)-4-oxo-2-piperidin-1-ylmethyl-3,4-dihydroquinazolin-6-ylmethyl]-N-(prop-2-ynyl)amino]benzoate (0.100 g, 0.17 mmol) (Preparation Example 32) in dichloromethane (2.5 ml) and trifluoroacetic acid (3.5 ml) was stirred at room temperature for 55 min with protection from the light. The solvent was then removed in vacuo and the residue was triturated with ether and dried in vacuo over P2O5. This solid was dissolved in anhydrous DMF (5 ml) under argon and the solution was placed in an ice-bath. A solution of 3-(aminomethyl)pyridine (0.027 g, 0.25 mmol) in DMF (0.2 nil) was then added followed by PyBOP® (0.092 g, 0.37 mmol) and diisopropylethylamine (0.197 g, 1.53 mmol). Stirring was continued at 0° C. for 15 min; then the ice-bath was removed and the reaction mixture was stirred for an additional 4 h at room temperature. The solvent was then removed in vacuo and the residue was chromatographed on aluminium oxide 90 active (neutral) column eluting first with chloroform and then with 1% methanol in chloroform. The product was obtained as a white solid (0.026 g, 24%), mp 90-93° C.; 1H-NMR (DMSO-d6) 1.40-1.49 (m, 6H, piperidine CH2CH2CH2), 2.20 (s, 6H, Me2N) 2.41 (m, 4H, piperidine CH2NCH2), 2.55 (t (obscured), 2H, Me2NCH2), 3.21(s, 1H, C≡CH), 3.62 (s, 2H, 2-CH2), 4.25 (t, J=7.0 Hz, 2H, N3-CH2), 4.38 (s, 2H, CH2C≡C), 4.45 (d, J=5.7 Hz, 2H, CONHCH 4.78 (s, 2H, 6-CH2), 6.78 (d, J=8.8 Hz, 2H, 3,5′-ArH), 7.32 (dd, J=4.8, 7.8 Hz, 1H, pyr 5-H), 7.68 (d, J=8.0 Hz, 1H, pyr 4-H), 7.75 (d, J=8.7 Hz, 2H, 2′,6′-ArH), 7.85, 7.91 (2×s, 2H, 5-H, 8-H), 8.43 (d, J=5.1 Hz, 1H, pyr 6-H), 8.52 (s, 1H, pyr 2-H), 8.74 (t, J=5.9 Hz, 1H, CONH).; MS (FAB, m/z) 626, 628 [(M+H)+, 100%, 35% respectively; Cl isotopic pattern]. FAB-HRMS: measured 626.3025; calculated for C35H41ClN7O2 (M+H)+: 626.3010.
