反应详情

A mixture of diethyl imidazole-4,5-dicarboxylate (2.65 g, 12.50 mmol) and ammonium sulfate (50 mg) was heated at reflux at 135° C. for 6 h with hexamethyldisilazine (50 mL). The reaction mixture was evaporated to dryness and the residue was co-evaporated twice with dry toluene (2×50 mL) to remove last traces of hexamethyldisilazine. The solid that obtained was dried in vacuo for 6 h and used for the next step without further characterization. To a solution of the above residue (12.5 mmol) in 1,2-dichlorethane (60 mL) was added 1-O-acetyl-2,3,5-tri-O-benzoyl-L-ribofuranose (5.06 g, 10 mmol) and tin tetrachloride (1.68 mL, 14.16 mmol) at 10° C. The reaction mixture was stirred under the atmosphere of argon at room temperature for 6 h. The reaction was checked by TLC using hexane and ethyl acetate (7:3). TLC indicated no starting material remained. The reaction mixture was diluted with dichloromethane (200 mL). The organic layer was washed with cold sat. sodium bicarbonate (200 mL) and brine (100 mL), dried over sodium sulfate and concentrated to yield a white foam. The residue was dissolved in dichloromethane (100 mL) and filtered through celite to remove tin salts. After evaporation in vacuo the residue (4.70 g) was dissolved in ethanol and filtered again through celite. The titled compound 47 was crystallized form the filtrate as needles. Yield 4.70 g (72%): mp 134-136° C.; 1H NMR (CDCl4) δ1.28 (t, 3H, CH3), 1.37 (t, 3H, CH3), 4.28-4.40 (m, 4H, 2CH2), 4.65-4.88 (m, 3H, C4′H and C5,H), 5.85 (m, 2H, C2′H and C3′H), 6.68 (d, 1H, C1′H, J1′,2′=3.90 Hz) and 7.26-8.08 (m, 16H, C2H and PhH).

1. temperaturewas heated
2. customThe reaction mixture was evaporated to dryness
3. customto remove last traces of hexamethyldisilazine
4. customThe solid that obtained
5. customwas dried in vacuo for 6 h
6. washThe organic layer was washed with cold sat. sodium bicarbonate (200 mL) and brine (100 mL)
7. dry with materialdried over sodium sulfate
8. concentrationconcentrated
9. customto yield a white foam
10. filtrationfiltered through celite
11. customto remove tin salts
12. customAfter evaporation in vacuo the residue (4.70 g)
13. dissolutionwas dissolved in ethanol
14. filtrationfiltered again through celite
15. customThe titled compound 47 was crystallized form the filtrate as needles