Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Hydrazine | HCID 9321 | — |
| Reactant | Uridine, 2′-O-[3-(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)propyl]-5-methyl-2-thio- | HCID 18660586 | — |
| Solvent | Methanol | HCID 887 | — |
| Product | HCID 18660584 | HCID 18660584 | — |
Reaction Steps
2′-O-Phthalimidopropyl-5-methyl-2-thiouridine (5.0 g, 15.8 mmol) is dissolved in 100 ml methanol in a 500 ml flask. Hydrazine (2.02 g, 63.2 mmol) is added and the mixture is heated to reflux (60-65° C.) with stirring for 14 hours. The solvent is evaporated in vacuo and the residue is dissolved in dichloromethane (150 ml) and extracted twice with an equal volume NH4OH. The organic layer is evaporated to yield the crude product. NMR is used to assay product purity. The product is used in subsequent reactions without further purification.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US06642367B2 |
|---|
| Source dataset | uspto-grants-2003_11 |
|---|
| ORD ID | ord-edfe9633ce5e459ebde537761a24c413 |
|---|
| Source note | uspto-grants-2003_11 |
|---|
