Water → HCID 9954958, compound

Participants

Reaction Steps

To a mixture of 5.064 g of 4-nitrobenzyl (4R,5S, 6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-3-{[(2R,3R)-2-({[(2S)-3-methyl-2-({[(4-nitrobenzyl)oxy]carbonyl}amino)-butanoyl]amino}methyl)tetrahydrofuran-3-yl]thio}-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate in 120 ml of ethyl acetate is added 5 ml of water, (about a 4% water:ethyl acetate biphasic solvent mixture) and after standing for about 5 minutes, gives a clear biphasic solvent mixture. An additional 55 ml of water is added followed by 2.5 g of 10% palladium-on-carbon. The reaction mixture is then hydrogenated in a Parr apparatus at 54 psi for 20 hours. The mixture is transferred to a separatory funnel and 100 ml of water is added. The aqueous layer is separated and the ethyl acetate layer washed with 20 ml of water. The combined aqueous layers are filtered through a pad of diatomaceous earth and the pad washed with an additional 30 ml of water. The combined aqueous layers are washed with 200 ml of ethyl acetate and lyophilized to afford 2.3 g(78%) of the desired product. MS (ES), m/z:442.3 (M+H)+, 98.9% area percent pure by analytical HPLC.

Provenance & Evidence