HuagongsheHuagongshe Chemistry Print Report · For research reference only
Sodium Chloride → Deoxycholic Acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Sodium Chloride | HCID 5234 | — |
| Reactant | sodium (4R)-4-[(3R,5R,8R,9R,10S,12S,13R,14S,17R)-3,12-dihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate | HCID 44582061 | — |
| Solvent | Tromethamine hydrochloride | HCID 93573 | — |
| Product | Deoxycholic Acid | HCID 222528 | — |
Reaction Steps
A pellet containing recombinant prothrombin immobilized on Ca3 (PO4)2 was obtained from a prothrombin-containing cell culture, as described above. Five 0.6 gram samples of moist adsorbate were suspended in 3 ml of 20 mM Tris-HCl buffer (pH 8.3) containing 50 mM, 100 mM, 250 mM, 350 mM or 500 mM sodium deoxycholate. Sixty microliter aliquots of 0.76 mg/ml trypsin were added to each sample, and the mixtures were incubated for three hours at room temperature with shaking. After incubation, the buffers of the samples were changed against 0.9% NaCl.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05792623 |
| Source dataset | uspto-grants-1998_08 |
| ORD ID | ord-ea5a9a7ae3ab403ea5a1905e0b16be7b |
| Source note | uspto-grants-1998_08 |