反应详情

5.0 gr of sphingosine (16.70 mM), prepared according to Example 4, 2.1 gr of N-acetylglycine (18.37 mM) and 3.8 gr of dicyclohexylcarbodiimide (18.40 mM) are left to react in 150 cc of chloroform, under warm conditions overnight. The reaction mixture is placed in a freezer to precipitate. The urea is filtered, and the chloroform solution is washed with H2O. The raw material is purified by chromatography using a mixture of CH2Cl2 /ethyl acetate/CH3OH, 70:30:10 v/v/v.

1. waitare left
2. customto precipitate
3. filtrationThe urea is filtered
4. washthe chloroform solution is washed with H2O
5. customThe raw material is purified by chromatography
6. additiona mixture of CH2Cl2 /ethyl acetate/CH3OH, 70:30:10 v/v/v