Water → HCID 178502830, compound

Participants

Reaction Steps

2.60 g of 7β-[2-(2-tritylaminothiazol-4-yl)-(Z)-2-methoxyiminoacetamido]-3-[(1-formamido-1H-tetrazol-5-yl)thiomethyl]-3-cephem-4-carboxylic acid were dissolved in 10 ml of 80% formic acid, and the solution was stirred at room temperature for one hour. 40 ml of water were added to the reaction solution, and insoluble materials were removed by filtration. The filtrate was condensed at a temperature below 40° C. under reduced pressure. One ml of tetrahydrofuran and 2 ml of ether were added to the residue, and the resultant precipitates were collected by filtration. 0.72 g of 7β-[2-(2-aminothiazol-4-yl)-(Z)-2-methoxyiminoacetamido]-3-[(1-formamido-1H-tetrazol-5-yl)thiomethyl]-3-cephem-4-carboxylic acid formate thus obtained as pale yellow powder were suspended in 8 ml of ethanol, and the suspension was stirred at 50° C. for 30 minutes. After cooling, the precipitates were collected by filtration. 0.53 g of 7β -[2-(2-aminothiazol-4-yl)-(Z)-2-methoxyiminoacetamido]-3-[(1-formamido-1H-tetrazol-5-yl)thiomethyl]-3cephem-4-carboxylic acid was thereby obtained as pale yellow powder.

Provenance & Evidence