HCID 141545489, compound → HCID 178502829, compound

Participants

Reaction Steps

2.92 g of 7β-[2-(2-tritylaminothiazol-4-yl)-(Z)-2-methoxyiminoacetamido]-3-[(1-benzylideneamino-1H-tetrazol-5-yl)thiomethyl]-3-cephem-4-carboxylic acid were dissolved in 15 ml of 80% formic acid, and the solution was stirred at room temperature for 2 hours. The precipitates were collected by filtration, washed with a mixture of 3 ml of isopropanol and 3 ml of ether, and then dried. 1.61 g of 7β-[2-(2-tritylaminothiazol-4-yl)-(Z)-2-methoxyiminoacetamido]-3-[(1-benzylideneamino-1H-tetrazol-5-yl)thiomethyl]-3-cephem-4-carboxylic acid formate thus obtained were dissolved in 20 ml of tetrahydrofuran. The resultant precipitates were collected by filtration, washed with isopropanol and ether, and then dried. The pale yellow powder thus obtained was dissolved in 15 ml of water. The aqueous solution was passed through the column packed with 50 ml of a styrene-divinylbenzene copolymer (Trade name "Diaion HP-20"). After the column was washed with water, said column was eluted with 20% aqueous ethanol. Then, the eluates obtained were freeze-dried. 1.01 g of 7β-[2-(2-aminothiazol-4-yl)-(Z)-2-methoxyiminoacetamido]-3-[(1-benzylideneamino-1H-tetrazol-5-yl)thiomethyl]-3-cephem-4-carboxylic acid sodium salt were thereby obtained as pale yellow powder.

Provenance & Evidence