HCID 53792923, compound → HCID 18652669, compound

Temperature

50 °C

500 mg 10% Pd/C was added to a solution of 4.5 g (8.60 mmoles) N-carbobenzyloxy-β-benzyl-L-aspartyl-D-alanine-1-cyclopentylethyl amide (from Part b) in 250 ml methanol. The mixture was hydrogenated at 40 psi for 4 hours, then the catalyst removed by filtration through a Celite® filter material. The Celite® filter material was then washed twice with 50 ml H2O and the combined methanol water filtrate concentrated to 100 ml in vacuo. This solution was diluted with 100 ml H2O, warmed to 50° C., and then filtered. The aqueous filtrate was freeze-dried to afford 2.55 g (8.5 mmoles) of L-aspartyl-D-alanine-1-cyclopentylethyl amide. HPLC analysis using a C-18 column and 20% CH3CN in 80% 0.2% KH2PO4 buffer pH 4.0 as eluant showed two peaks with a ratio of 6:1, representing the S and R isomers of the cyclopentylethyl amides. Three recrystallizations of the product from 95% ethanol gave 610 mg of the pure L-aspartyl-D-alanine-(S)-1-cyclopentylethyl amide, mp 204°-5° C.

1. customthe catalyst removed by filtration through a Celite®
2. filtrationfilter material
3. filtrationThe Celite® filter material
4. washwas then washed twice with 50 ml H2O
5. concentrationthe combined methanol water filtrate concentrated to 100 ml in vacuo
6. additionThis solution was diluted with 100 ml H2O
7. filtrationfiltered
8. customThe aqueous filtrate was freeze-dried

ORD record

ord-120f6990845b441e849a6691bb43ca23

Source dataset

uspto-grants-1993_07

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05225591