反应详情

Methyl 6-{4-[(N-acetyl-4-{{2-[(benzhydryloxy)carbonyl]phenyl}[(benzyloxy)(oxo)acetyl]amino}-3-ethylphenylalanyl)amino]butoxy}-3-bromo-2-hydroxybenzoate was treated with trifluoroacetic acid (500 μL)/methylene chloride (500 μL) at ambient temperature for 4 hours, concentrated under reduced pressure and co-evaporated with acetonitrile (2×10 mL). The residue was taken up in 1N NaOH (3 eq.)/methanol (250 μL)/THF (250 μL), stirred for 3 hours and concentrated under reduced pressure to provide the titled compound. MS (ESI (+)) m/e 742, 743 (M+H)+; 1H NMR (500 MHz, DMSO-d6) 8.08-7.92 (m, 2H), 7.45-6.94 (m, 7H), 6.64 (d, 2H), 4.43-4.38 (m, 1H), 3.90-3.86 (m, 2H), 3.78 (s, 3H), 3.10-3.05 (m, 2H), 2.90-2.85 (m, 1H), 2.75-2.62(m, 3H), 1.76(s, 3H), 1.64-1.58 (m, 2H), 1.52-1.45 (m, 2H), 1.18 (t, 3H).

1. concentrationconcentrated under reduced pressure and co-evaporated with acetonitrile (2×10 mL)
2. concentrationconcentrated under reduced pressure