Dimethylformamide → N'-(Cyclobutylcarbonyl)-1-ethyl(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide

Participants

Reaction Conditions

Reaction Steps

TBTU (0.050 g, 0.15 mmol) and diisopropylethylamine (0.04 ml, 0.26 mmol) in DMF (0.5 ml) was added to cyclobutylcarboxylic acid (RYCOOH, 0.015 g, 0.15 mmol). The reaction mixture was stirred for 40 minutes at 20° C. A mixture of Intermediate 19 (0.045 g, 0.13 mmol) and diisopropylethylamine (0.04 ml, 0.26 mmol) in DMF (0.5 ml) was added and the reaction mixture stirred for 18 h. The solvent was removed in vacuo and the residue applied to a SPE cartridge (aminopropyl, 2 g). The cartridge was eluted with methanol to afford Intermediate 65 (0.052 g). LCMS showed MH+=387; TRET=2.28 min.

Provenance & Evidence