反应详情

In accordance with the process described in JP Patent Application Laying Open (Kokai) No. 62-55097, Enterobacter cloacae N-7901 (FERM BP-873) was cultured. 1 L of culture solution was centrifuged, and the wet cells were then suspended in distilled water to prepare a 800 g cell suspension. 200 g of D,L-tert-leucine amide was dissolved in this suspension, and the solution was then allowed to react at 40° C. for 52 hours. After the reaction, cells were removed by centrifugation and 970 g of an aqueous solution containing 10% by mass each of L-tert-leucine and D-tert-leucine amide was obtained.

1. customto prepare a 800 g cell suspension
2. customto react at 40° C. for 52 hours
3. customAfter the reaction, cells
4. customwere removed by centrifugation and 970 g of an aqueous solution
5. additioncontaining 10% by mass each of L-tert-leucine