反应详情

温度

-70 °C

10.6 g (0.025 mmol) of 5-(2-phtalimidoethyl)-1-(β-D-ribopyranosyl)uracil were dissolved in 20 ml of pyridine in a heated and argon-flushed 1 l four-necked flask and mixed with 200 ml of dichloromethane. The mixture was cooled to −70° C., 3.82 ml (0.033 mmol) of benzoyl chloride in 5 ml of pyridine and 20 ml of dichloromethane were slowly added dropwise with cooling and the mixture was stirred at −70° C. for 35 min. The reaction mixture was poured onto 600 ml of cooled ammonium chloride solution and the aqueous phase was extracted with ethyl acetate. The combined organic phases were washed with water, dried and concentrated to dryness in vacuo. Chromatography on silica gel (ethyl acetate/heptane 1:1) yielded 7.9 g (60%) of 1-(2′-O-benzoyl-β-D-ribopyranosyl)-5-(2-phtalimido-ethyl)uracil.

1. temperaturea heated
2. customargon-flushed 1 l four-necked flask
3. additionmixed with 200 ml of dichloromethane
4. temperaturewith cooling
5. extractionthe aqueous phase was extracted with ethyl acetate
6. washThe combined organic phases were washed with water
7. customdried
8. concentrationconcentrated to dryness in vacuo