Water → HCID 57750143, compound

Participants

Reaction Steps

At room temperature, a mixture of chiral (2′R,3R,4′S)-1-acetyl-6-chloro-4′-[5-chloro-2-(1-ethyl-1-methanesulfonylaminocarbonyl-propoxy)-phenyl]-2′-(5-chloro-2-methyl-phenyl)-spiro[3H-indole-3,3′-piperidine]-2,6′(1H)-dione (20 mg, 0.027 mmol) (RO5319796-000), NaOH (2 mg, 0.05 mmol), H2O (0.5 mL) and methanol (2 mL) was stirred overnight. Then methanol was removed by vacuum. The aqueous solution was acidified by addition of concentrated HCl to “pH” 1-2 and extracted with EtOAc twice. The combined organic layers were dried over anhydrous Na2SO4 and concentrated to give the title compound as a white solid (10 mg).

Provenance & Evidence