HCID 66613551, compound → HCID 178518777, compound

Participants

Reaction Steps

500 mg (1.291 mmol) of the compound from example 68A were admixed with 7 ml of acetic acid and 273 mg (1.291 mmol) of 3,4-diamino-1H-pyrazol-5-ol sulfate. The mixture was stirred at RT for 16 h and then concentrated on a rotary evaporator. Water and ethyl acetate were added, and the mixture was stirred. The organic phase was removed and extracted once with a 1N sodium hydroxide solution, dried over sodium sulfate and concentrated on a rotary evaporator. The residue was purified by preparative HPLC (eluent: water/acetonitrile/water with 1% trifluoroacetic acid, ratio 80:15:5). 142 mg of the title compound were obtained (29% of theory).

Provenance & Evidence