反应详情

温度

60 °C

Solutions of the individual diastereoisomers of tert-butyl {1-[5-chloro-2-methoxy-4-methyl-3-(5-oxopyrrolidin-3-yl)phenyl]ethyl}carbamate (0.31 g, 0.80 mmol [peak 1 from Examples 192, step 3]; 0.31 g, 0.80 mmol [peak 2 from Examples 192, step 3]) in DMF (4 mL) at 0° C. were each treated individually with sodium hydride dispersed in mineral oil (80 mg, 2.0 mmol). The ice bath was removed and the reaction mixtures were stirred for 30 min and heated at 60° C. for 30 minutes. The reaction mixtures were cooled down to 0° C., treated with methyl iodide (0.060 mL, 0.96 mmol) in DMF (2 mL, 26 mmol), and stirred at room temperature for 16 hours. The reaction mixtures were cooled to 0° C., quenched with saturated ammonium chloride, and extracted with EtOAc. The organic extract was concentrated to give a crude oil which was purified by preparative LCMS (XBridge C18 column, eluting with a gradient of acetonitrile/water containing 0.1% ammonium hydroxide, at flow rate of 60 mL/min) to give diastereoisomer from peak 1 (28 mg, 9%, retention time: 2.52 min) and diastereoisomer from peak 2 (56 mg, 18%, retention time: 2.51 min). Peak 1: LCMS for C20H29ClN2O4Na (M+Na)+: m/z=419.2. Found: 419.1. Peak 2: LCMS for C20H29ClN2O4Na (M+Na)+: m/z=419.2. Found: 419.1.

1. customat 0° C.
2. customThe ice bath was removed
3. customthe reaction mixtures
4. customThe reaction mixtures
5. temperaturewere cooled down to 0° C.
6. stirringstirred at room temperature for 16 hours
7. customThe reaction mixtures
8. temperaturewere cooled to 0° C.
9. customquenched with saturated ammonium chloride
10. extractionextracted with EtOAc
11. extractionThe organic extract
12. concentrationwas concentrated
13. customto give a crude oil which
14. customwas purified by preparative LCMS (XBridge C18 column
15. washeluting with a gradient of acetonitrile/water containing 0.1% ammonium hydroxide, at flow rate of 60 mL/min)
16. customto give diastereoisomer from peak 1 (28 mg, 9%, retention time: 2.52 min) and diastereoisomer from peak 2 (56 mg, 18%, retention time: 2.51 min)