Sulfuric acid magnesium salt (1:1) → 7-[(3aS,4R,6R,6aR)-6-{[({3-[(5-tert-butyl-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-1,3-benzodiazol-2-yl)methyl]cyclobutyl}methyl)(propan-2-yl)amino]methyl}-2,2-dimethyl-hexahydrocyclopenta[d][1,3]dioxol-4-yl]-7H-pyrrolo[2,3-d]pyrimidin-4-amine

Participants

Reaction Steps

3-[(5-Tert-butyl-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-1,3-benzodiazol-2-yl)methyl]cyclobutane-1-carbaldehyde (282.3 mg, 0.7 mmol), 7-[(3aS,4R,6R,6aR)-2,2-dimethyl-6-[(propan-2-ylamino)methyl]-hexahydrocyclopenta[d][1,3]dioxol-4-yl]-7H-pyrrolo[2,3-d]pyrimidin-4-amine (243.41 mg, 0.7 mmol) and magnesium sulfate (127.22 mg, 1.06 mmol) were stirred in 1,2-dichloroethane (10 ml) for 15 mins. Sodium triacetoxyborohydride (179.21 mg, 0.85 mmol) was added, and the reaction stirred overnight. The reaction was quenched by the addition of sat. Na2CO3 (10 ml), and the solution was extracted with DCM (3×10 ml). The combined organics were dried (MgSO4) and concentrated. The crude material was purified by mass directed prep-HPLC (acidic method). After combining the fractions, a small amount of 7M NH3 in MeOH was added to basify the solution. After concentration, the residue was partitioned between water (5 ml) and DCM (5 ml), and the layers separated. The aqueous layer was extracted with DCM (2×3 ml) and the combined organics were dried (MgSO4) and concentrated to afford the title compound (58.7 mg, 11%) as a colourless gum: MS (ESI) for C41H63N7O3Si m/z 730.2 [M+H]+; LC purity 95% (UV), 100% (ELS), (ret. time, 1.65 min); 1H NMR (500 MHz, CHLOROFORM-d) δH 8.30 (s, 1H), 7.75 (s, 1H), 7.32 (s, 2H), 7.04 (d, J=3.6 Hz, 1H), 6.37 (d, J=3.6 Hz, 1H), 5.40-5.50 (m, 2H), 5.24 (br. s., 2H), 4.89-5.04 (m, 2H), 4.45 (d, J=5.2 Hz, 1H), 3.50 (s, 3H), 2.11-3.09 (m, 13H), 2.01 (s, 6H), 1.34-1.49 (m, 6H), 1.29 (s, 3H), 0.85-1.02 (m, 8H), −0.11-0.05 (m, 9H).

Provenance & Evidence