反应详情

N-(N′-Boc-3′-aminopropyl)-N-[(1-thyminyl)acetyl]glycine methyl ester (3.02 g, 0.00732 mol) was dissolved in methanol (25 ml) and stirred for 1.5 h with 2 M sodium hydroxide (25 ml). The methanol was removed by evaporation, in vacuo, and pH adjusted to 2 with 4 M hydrochloric acid at 0° C. The product was isolated as white crystals by filtration, washed with water (3×10 ml), and dried over sicapent, in vacuo. Yield 2.19 g (75%). Anal. for C17H26N4O7, H2O, found (calc.) C, 49.95; (49.03); H, 6.47; (6.29); N, 13.43; (13.45). The compound showed satisfactory 1H and 13C-NMR spectra.

1. customThe methanol was removed by evaporation, in vacuo
2. custompH adjusted to 2 with 4 M hydrochloric acid at 0° C
3. customThe product was isolated as white crystals by filtration
4. washwashed with water (3×10 ml)
5. customdried over sicapent, in vacuo