反应详情

温度

95 °C

Samples were diluted to 1 mg/ml with the relevant buffer and 16.71a1 added to 12.5 μl of 4×LDS sample buffer (Invitrogen), 15.8 μl of Milli-Q water and 5 μl reducing agent (Invitrogen). The samples were heated at 95° C. for one minute and then placed on ice. 15 μl of each sample was loaded onto a 4-12% BisTris gel (Invitrogen) in a tank containing 1×MES SDS running buffer and the gel run for 35 minutes at a constant current of 500 mA. After electrophoresis the gel was removed from its casing, rinsed for 3×10 minutes with Milli-Q water, stained with Gelcode® Blue staining reagent (Pierce) for a minimum of one hour and then destained with Milli-Q water. The gel was photographed and analysed using a UVP GDS8000 gel documentation system. The relative abundance of BAK502G9 heavy and light chain in each sample was determined.

1. additionSamples were diluted to 1 mg/ml with the relevant buffer and 16.71a1
2. customwas removed from its casing
3. washrinsed for 3×10 minutes with Milli-Q water
4. customstained with Gelcode® Blue staining reagent (Pierce) for a minimum of one hour