Hydrochloric Acid → HCID 89690135, compound

Participants

Reaction Steps

To a solution of ethyl 7-(8-hydroxy-8-methyl-2,3-di-p-tolyl-7,8-dihydropyrido[3,2-b]pyrazin-5(6H)-yl)heptanoate (step 1) (10 mg, 0.020 mmol) in EtOH (1 ml) was added 2M NaOH (aq) (0.040 ml, 0.080 mmol). The reaction mixture was stirred at RT overnight under an atmosphere of nitrogen. A further portion of 2M NaOH (aq) (0.040 ml, 0.080 mmol) was added and stirring continued overnight. The resulting mixture was treated with 2M HCl (aq) to adjust the pH to pH5. The organic solvent was removed under reduced pressure and the mixture was diluted with water (10 ml) and extracted with ethyl acetate (2×10 ml). The combined extracts were dried over MgSO4, filtered and concentrated under reduced pressure. The crude product was purified by chromatography on silica eluting with iso-hexane/EtOAc to afford the titled compound;

Provenance & Evidence