2-Chloropyridine → HCID 178457802, compound

Participants

Reaction Steps

A microwave vial was charged with 4-fluoro-N-(6-hydroxy-1-(cis-4-(isopropylcarbamoyl)cyclohexyl)-1H-benzo[d]imidazol-2-yl)benzamide (56 mg, 0.128 mmol), 2-chloropyridine (0.036 mL, 0.383 mmol), cesium carbonate (166 mg, 0.511 mmol), and NMP (0.6 mL) then irradiated in the microwave at 160° C. for 6 hours. The mixture was diluted with EtOAc, washed with water, the water layer back extracted with EtOAc, and the combined organics were washed with brine. The organic layer was dried over Na2SO4 and filtered. The mixture was purified via flash chromatography using a linear gradient of 0% to 10% MeOH in DCM. The material was further purified by reverse-phase preparative HPLC using a 0.1% TFA in ACN/H2O, gradient 10% to 90%. The material was partitioned between saturated aqueous NaHCO3 and 5% IPA/DCM, the organic layer washed with brine, dried over Na2SO4, filtered and evaporated. The title compound was isolated as an off-white solid (32 mg, 35% yield). 1H NMR (400 MHz, DMSO-d6) δ ppm 0.98 (d, J=8.00 Hz, 6H) 1.54-1.82 (m, 4H) 2.01-2.14 (m, 2H) 2.42-2.56 (m, 1H) 2.56-2.77 (m, 2H) 3.75-3.93 (m, 1H) 4.80-4.95 (m, 1H) 6.97 (dd, J=8.56, 2.10 Hz, 1H) 6.95-7.10 (m, 1H) 7.00-7.18 (m, 1H) 7.20-7.29 (m, 2H) 7.48 (d, J=2.05 Hz, 1H) 7.51-7.62 (m, 2H) 7.80-7.90 (m, 1H) 8.07-8.19 (m, 1H) 8.27-8.38 (m, 2H) 12.81 (s, 1H). M/Z calc'd for C29H30FN5O3: 515.58. found 516 [M+H].

Provenance & Evidence