反应详情

Sodium hydroxide (2 ml, 4.00 mmol) was added to a solution of N-[5-[4-(5-{[(2R,6S)-2,6-dimethyl-4-morpholinyl]methyl}-1,3,4-oxadiazol-2-yl)-1-(phenylsulfonyl)-1H-indazol-6-yl]-2-(methyloxy)-3-pyridinyl]-2,4-difluorobenzenesulfonamide (194 mg, 0.212 mmol) in 1,4-dioxane (2 ml). The mixture was stirred at room temperature for 2 h. The solvent was evaporated under a stream of nitrogen and the residue partitioned between ethyl acetate (10 ml) and saturated ammonium chloride (5 ml). The organic layer was separated by hydrophobic frit, washed with water and the solvent removed in vacuo. The residual solid was purified by Mass Directed Automated Preparative HPLC (Method B) and the solvent was evaporated under a stream of nitrogen to give the title compound as a cream coloured solid (10 mg).

1. customThe solvent was evaporated under a stream of nitrogen
2. customthe residue partitioned between ethyl acetate (10 ml) and saturated ammonium chloride (5 ml)
3. customThe organic layer was separated by hydrophobic frit
4. washwashed with water
5. customthe solvent removed in vacuo
6. customThe residual solid was purified by Mass Directed Automated Preparative HPLC (Method B)
7. customthe solvent was evaporated under a stream of nitrogen