Reaction Steps
To a solution of 1-tert-butyl 4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-((E)-3-oxo-3-((1-(pyrimidin-2-yl)cyclopropyl)amino)prop-1-en-1-yl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (460 mg, 0.576 mmol) in dichloromethane (6 mL) was added TFA (3 mL, 0.576 mmol). The reaction mixture was stirred at r.t for 2 hs and evaporated in vacuo to afford crude product, which was purified by preparative-HPLC (Mobile Phase: A=0.05% TFA/H2O, B=MeCN) to give the title compound, trifluoroacetic acid salt (100 mg, 0.116 mmol, 20.19%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ=12.18 (br. s., 1H), 8.78-8.57 (m, 3H), 7.39-7.15 (m, 1H), 6.68 (d, J=16.1 Hz, 1H), 5.94 (d, J=16.1 Hz, 1H), 4.40 (dd, J=4.6, 11.2 Hz, 1H), 3.30-3.11 (m, 1H), 2.90-2.67 (m, 1H), 2.59-2.52 (m, 1H), 2.28-2.01 (m, 3H), 2.01-0.75 (m, 49H); LC/MS: m/z calculated 741.5. Found 742.4 (M+1)+.
