反应详情

N-Bromosuccinimide (1.504 g, 8.45 mmol) was added portionwise to a stirred solution of 2-{[(1S)-1-methylpentyl]oxy}-9-(tetrahydro-2H-pyran-2-yl)-9H-purin-6-amine (2.453 g, 7.68 mmol) in chloroform (40 ml) under at atmosphere of nitrogen cooled in an ice-bath. After 3 hours LCMS indicated the reaction to be 80% complete and more N-bromosuccinimide (0.68 g) was added and stirring continued for a further 2 hours. Water (40 ml) was added and the phases separated using a hydrophobic frit. The organic phase was evaporated and the residue dissolved in dichloromethane and purified on an aminopropyl (NH2) cartridge (100 g) using a 0-100% ethyl acetate in cyclohexane gradient followed by a 0-20% methanol (+1% triethylamine) gradient over 60 mins. The appropriate fractions were combined and evaporated in vacuo to the title compound as a white foam (2.38 g).

1. temperaturecooled in an ice-bath
2. customthe reaction
3. waitcontinued for a further 2 hours
4. customthe phases separated
5. customThe organic phase was evaporated
6. dissolutionthe residue dissolved in dichloromethane
7. custompurified on an aminopropyl (NH2) cartridge (100 g)
8. waitfollowed by a 0-20% methanol (+1% triethylamine) gradient over 60 mins
9. customevaporated in vacuo to the title compound as a white foam (2.38 g)