Sodium hydride → (4S,5R)-5-[3,5-Bis(trifluoromethyl)phenyl]-3-({5-[5-(3,5-dimethyl-1-propyl-1H-pyrazol-4-yl)-2-methoxypyridin-3-yl]-2-(3-fluoroazetidin-1-yl)pyrimidin-4-yl}methyl)-4-methyl-1,3-oxazolidin-2-one

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Reaction Steps

To a solution of (4S,5R)-5-[3,5-Bis(trifluoromethyl)phenyl]-3-({5-[5-(3,5-dimethyl-1H-pyrazol-4-yl)-2-methoxypyridin-3-yl]-2-(3-fluoroazetidin-1-yl)pyrimidin-4-yl}methyl)-4-methyl-1,3-oxazolidin-2-one (EXAMPLE 17, 0.020 g, 0.029 mmol) in THF (2 mL) was added sodium hydride (0.011 g, 0.29 mmol) at 0° C. The resulting mixture was stirred for 10 minutes at room temperature, after which iodopropane (0.075 g, 0.044 mmol) was added. The resulting mixture was stirred for 24 hours at room temperature until the starting material was consumed completely. Water (1 mL) was added to quench the reaction. The aqueous layer was extracted with Ethyl acetate (2×2 mL), the combined organic layer was concentrated in vacuum and the residue was dissolved in 1 mL of DMSO and filtered. This clear DMSO filtrate was purified by preparative HPLC (reverse phase, Waters SunFire Prep C18 OBD5 um 19×100 mm) eluting with acetonitrile/water+0.14% HCOOH (30% to 100% organic in 5 minutes, 25 mL/minutes). Related fractions were pooled and evaporated under reduced pressure to afford a fluffy white solid of 2.8 mg as the titled compound. LCMS (M+H)+: 722.4. 1H NMR (DMSO-d6, 500 MHz): δ 8.24 (s, 1H), 8.16-8.14 (d, J=8.5 Hz, 2H), 7.92 (s, 2H), 7.62 (s, 1H), 5.87 (d, J=8.5 Hz, 1H), 5.60 (s, 1H), 5.42 (s, 1H), 4.53-3.98 (br m, 4H), 3.84 (s, 3H), 2.52 (s, 3H), 2.10 (m, 3H), 2.04 (m, 2H), 1.68 (m, 1H), 1.60-1.44 (m, 2H), 0.83 (t, J=6.5 Hz, 3H), 0.54 (d, J=6.5 Hz, 3H). RTA IC50: 12 nM

Provenance & Evidence