Glycolaldehyde dimer → HCID 178479700, compound

Participants

Reaction Steps

Sodium cyanoborohydride (0.011 g, 0.18 mmol) was added to a solution of the product from Example 10.2 (0.041 g, 0.15 mmol) and 1,4-dioxane-2,5-diol (0.027 g, 0.22 mmol, Sigma-Aldrich) in MeOH (3 ml) and stirred at it for 16 h. The solvent was evaporated and the residue chromatographed on silica gel (iPrOH-28% aq. NH4OH-water, 98:1:1) to give the crude product as a yellow solid (˜21 mg). The solid was triturated with a little 7M NH3-EtOH solution to leave the free base form of the title compound as a colourless solid (˜12 mg). This solid was dissolved in excess 5% aq. HCl then the solvent evaporated to give 7-(((1,3-dihydroxypropan-2-yl)(2-hydroxyethyl)amino)methyl)-3H-pyrrolo[3,2-d]pyrimidin-4(5H)-one hydrochloride as a solid (15 mg, 32%). 1H NMR (D2O, referenced to HOD at 4.72 ppm) δ 8.49 (s, 1H), 7.82 (s, 1H), 4.77 (s, 2H), 3.98-3.88 (m, 6H), 3.77 (sextet, J=6.1 Hz, 1H), 3.56 (br. s, 2H). 13C NMR (D2O), δ 154.4, 144.4, 139.5, 132.8, 118.6, 104.1, 64.3, 56.7, 55.7, 52.6, 47.1. +ESMS Found 283.1407 (M+H)+ C12H19N4O4 requires 283.1406.

Provenance & Evidence