Hydrochloric Acid → N2-isoButyryl-9-carboxymethylguanine

Participants

Reaction Steps

Compound 11 (3.07 g, 10 mmol) and NaOH (0.8 g, 20 mmol) were dissolved in water (20 mL). The mixture was stirred at room temperature for 2 h. The pH was adjusted to 3 using 1M HCl, to obtain a white precipitate. The solution was filtered off, and the precipitate was washed with a small amount of cold water and dried in vacuo to give 5 as a white solid: yield 2.65 g (95%); MS(+ESI): m/z 280 [M+H]+; 1H NMR (400 MHz, d-DMSO): δ13.32 (s, 1H), 12.06 (s, 1H), 11.64 (s, 1H), 7.93 (s, 1H), 4.88 (s, 2H), 2.74 (septet, 1H, J=6.8 Hz), 1.10 (d, 6H, J=6.8 Hz); 13C NMR (400 MHz, CDCl3): δ180.63, 169.52, 155.29, 149.47, 148.52, 140.78, 120.17, 44.88, 39.38, 19.33.

Provenance & Evidence