Sodium Cyanide → HCID 25013535, compound

Temperature

21 °C

To a 100-mL flask was added 40 mL of dH2O and 1.8 mL of 6,6-dimethyl-3-azabicyclo[3.1.0]hexane to provide a homogeneous solution with a pH of approximately 11.4. To this solution was added 2.7 g of Na2S2O5 to provide a homogeneous solution and the pH was adjusted to about 7.5 with approximately 1 mL of 8N NaOH. To this solution was added 60 μL of Antifoam-204 (Sigma catalog number A-6226),120 μL of A. niger catalase suspension (Sigma Aldrich; catalog number C-3515) and 300 mg of monoamine oxidase powder of the polypeptide of SEQ ID NO 8 (prepared by the method of Example 2) in 10 mL of 100 mM pH 8.0 potassium phosphate buffer. Air was sparged into the reaction mixture through fritted glass at a rate of ˜10 mL/min and the resulting pale yellow solution was stirred under air for 24 hours at room temperature (about 21° C.) for 24 hours. Throughout, the pH was maintained at 7.4 via feedback controlled addition of 2.5N NaOH in 1 μL portions. After 24 hours, 1.0 g (1.3 equiv) of NaCN was added to the reaction mixture then comprising (1R,2S,5S)-6,6-dimethyl-3-azabicyclo[3.1.0]hexane-2-sulfonate. After stirring at room temperature (about 21° C.) for an additional 15 minutes, the mixture was extracted with MTBE. (2-Me-THF can also be used for the extraction.) Following phase separation and solvent removal, 1.78 g (90% yield) of (1R,25,55)-6,6-dimethyl-3-azabicyclo[3.1.0]hexane-2-carbonitrile was isolated a pale yellow solid. Analysis by 1H-NMR in CDCl3 confirmed preparation of (1R,2S,5S)-6,6-dimethyl-3-azabicyclo[3.1.0]hexane-2-carbonitrile (1H-NMR (300 MHz, CDCl3) spectrum: δ 3.92 (d, J=1.2, 1H, NC(H)(CN)), 3.25 (m, 1H), 2.96 (dd, J=2.1, 17.0), 1.48 (dd; J=1.2, 12.2; 1H), 1.42 (m, 1H), 1.13 (s, 3H), 1.11 (S, 3H)).

1. customto provide a homogeneous solution with a pH of approximately 11.4
2. customto provide a homogeneous solution
3. additionTo this solution was added 60 μL of Antifoam-204 (Sigma catalog number A-6226),120 μL of A
4. customAir was sparged into the reaction mixture through fritted glass at a rate of ˜10 mL/min
5. temperatureThroughout, the pH was maintained at 7.4 via feedback
6. additionaddition of 2.5N NaOH in 1 μL portions
7. waitAfter 24 hours
8. stirringAfter stirring at room temperature (about 21° C.) for an additional 15 minutes
9. extractionthe mixture was extracted with MTBE
10. extraction(2-Me-THF can also be used for the extraction
11. customphase separation and solvent removal

ORD record

ord-4f3e9710a26841d1ae58f1eccaa09930

Source dataset

uspto-grants-2014_10

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08859784B2