Sodium Hydroxide → HCID 52914570, compound

Participants

Reaction Steps

2 N Aqueous sodium hydroxide (5.5 mL) was added to methyl 4-(2-(4-cyano-1-((2R,3S)-2-propyl-1-(3-(trifluoromethyl)picolinoyl)-3-(5-(trifluoromethyl)thiophen-3-yloxy)piperidine-3-carbonyl)piperidin-4-yl)benzyloxy)-2,2-dimethylbutanoate (312 mg, 0.37 mmol) in THF/methanol (1:3, 12 mL). The reaction was stirred for 8 h at room temperature then concentrated. The residue was diluted ethyl acetate (30 mL) and the pH adjusted to 1 with 1 N hydrochloric acid. The organic layer was washed with brine, dried over Na2SO4, filtered and evaporated to dryness. The residue was purified by C18 semi-prep HPLC to obtain A60 (300 mg, 99% yield) as a white powder after lyophilization from water/acetonitrile: 1H NMR (CDCl3, 300 MHz) δ 8.81-8.82 (d, J=3.9 Hz, 1H), 8.06-8.08 (d, J=8.1 Hz, 1H), 7.24-7.50 (m, 5H), 6.84-6.87 (d, J=8.1 Hz, 1H), 6.53-6.56 (m, 1H), 4.65-5.57 (m, 5H), 3.48-3.75 (m, 6H), 3.08-3.20 (m, 3H), 1.22 (s, 6H), 0.93-2.47 (m, 17H).

Provenance & Evidence