Magnesium Sulfate Heptahydrate → L-Aspartic Acid

関与物質

反応手順

Serratia marcescens TA5002 was inoculated in a medium (500 ml, pH 7.0) containing ammonium fumarate (3%), potassium dihydrogen phosphate (0.2%), magnesium sulfate heptahydrate (0.05%), corn steep liquor (4%) and yeast extract (2%) and cultivated at 30° C. for 18 hours. The resulting culture was adjusted to pH 8.5 with aqueous ammonia and added thereto ammonium fumarate (65 g) and Triton X-100 (500 mg). The mixture was allowed to react at 37° C. for 5 hours. Then, the reaction mixture was filtered, concentrated and adjusted to pH 2.8 to precipitate crude crystals of L-aspartic acid. The crude crystals were filtered off and recrystallized from water to give L-aspartic acid (46.5 g).

情報源と証拠