HuagongsheHuagongshe Chemistry Print Report · For research reference only
Chloroform → 1,2-Dioleoyl-sn-glycero-3-phosphocholine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Chloroform | HCID 6212 | — |
| Reactant | N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium | HCID 6438350 | — |
| Reactant | 1,2-Dioleoyl-sn-glycero-3-phosphocholine | HCID 10350317 | — |
| Product | 1,2-Dioleoyl-sn-glycero-3-phosphocholine | HCID 10350317 | — |
| Product | Dotma dopc | HCID 131716290 | — |
Reaction Steps
DOTMA/DOPC SUV's were prepared by dissolving 40 mg DOTMA and 60 mg DOPC together in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 2 ml water with vigorous shaking and sonicated in a bath type sonicator for 60 minutes until clear, producing DOTHA/DOPC vesicles at a concentration of 50 mg/ml.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05622712 |
| Source dataset | uspto-grants-1997_04 |
| ORD ID | ord-4b47fa3023224dd4ad90ab028285444d |
| Source note | uspto-grants-1997_04 |