反应详情

For the partitioning experiments described below, calibration curves of dansyl ethanol in water and octanol respectively were initially made (see FIGS. 5A-5B). The UV-vis spectra were recorded using an Agilent 8453 spectrophotometer (Agilent, Inc., Santa Clara, Calif.) at λ=335 nm. From the calibration curves, ideal oligomer concentrations were made, which allowed absorbance between 1.0-1.5 instrumentation absorbance units (AU), corresponding to initial oligomer concentrations of ˜0.1 mM (in water). After the oligomer was dissolved in water (1 mL), octanol (1 mL) was added and the UV-spectra recorded in both the water and octanol layer. Sodium laurate (1.2 eq to the total guanidine concentration) was added, the vial gently shaken, and the UV spectra recorded in both the water and octanol layer. Following partitioning the water and octanol layers were separated after which the aqueous phase was lyophilized and its contents analyzed with 1H-NMR.

1. concentrationFrom the calibration curves, ideal oligomer concentrations
2. dissolutionAfter the oligomer was dissolved in water (1 mL)
3. additionoctanol (1 mL) was added
4. concentrationSodium laurate (1.2 eq to the total guanidine concentration)
5. additionwas added
6. customFollowing partitioning the water and octanol layers
7. customwere separated after which the aqueous phase