Doxorubicin Hydrochloride → Doxorubicin

Participants

Reaction Steps

100 μl of n-butylcyanoacrylate monomer was added to 1% dextran solution in 8 mL of 0.01 M HCl under constant stirring at 600 rpm. Doxorubicin hydrochloride powder 50 mg was dissolved in 2 mL of distilled water and after 40 minutes the solution was added to the reaction flask. After 4 hours the pH of the nanoparticle suspension was adjusted at 7 and filtered through 0.8 μm membrane filter (Nuclepore Track-Etch membrane, Whatman, USA). The doxorubicin-loaded nanoparticle suspension was kept in a refrigerator at 4° C.

Provenance & Evidence