反应详情

3-[4-(4′,5′-Difluoro-2′-methoxy-biphenyl-4-yloxymethyl)-indazol-1-yl]-propionic acid ethyl ester (0.095 g, 0.204 mmol) and lithium hydroxide hydrate (10 mg, 0.245 mmol) in 5 mL THF/1 mL H2O was stirred at RT for 3 hrs. The reaction was distributed between EtOAc and H2O. The water layer was made acidic to pH 3 with 1N HCl and the organic layer was separated and concentrated in vacuo. The crude product was purified by flash chromatography with a gradient from 0-3% methanol in CH2Cl2 to yield 3-[4-(4′,5′-difluoro-2′-methoxy-biphenyl-4-yloxymethyl)-indazol-1-yl]-propionic acid as a white solid (53 mg, 59%). LC-MS (ES) calculated for C24H20F2N2O04, 438.43; found m/z 439 [M+H]+.

1. customthe organic layer was separated
2. concentrationconcentrated in vacuo
3. customThe crude product was purified by flash chromatography with a gradient from 0-3% methanol in CH2Cl2