反应详情

A solution of 3(S)-(6-chloro-9H-β-carbolin-8-ylcarbamoyl)-morpholine-4-carboxylic acid tert-butyl ester (1.00 g, 2.32 mmol) in CH2Cl2 (6 ml) was stirred at RT. Trifluoroacetic acid (6 ml) was added and the solution was stirred at RT for 45 min, then concentrated to a residue. The residue was concentrated once more from CH2Cl2 to yield a yellow-brown solid which was dissolved in THF (13 ml) under argon. Gentle warming was sometimes needed to ensure complete dissolution. A solution of N-(tert-butoxycarbonyl)-D-prolinal (693 mg, 3.48 mmol) in THF (2 ml) was added, followed by sodium triacetoxyborohydride (738 mg, 3.48 mmol). The solution was stirred at RT for 30 min, then quenched via addition of 1N aqueous NaOH (30 ml). The mixture was poured into a separatory funnel containing EtOAc (100 ml), H2O (100 ml), and brine (100 ml). The mixture was shaken and the layers were separated. The aqueous layer was extracted with EtOAc (2×50 ml) and the combined organic layers were washed with brine. The organic layer was dried, filtered and concentrated to yield a light brown solid. Column chromatography (2%-4% MeOH/CH2Cl2) yielded 2(R)-[3(S)-(6-Chloro-9H-β-carbolin-8-ylcarbamoyl)-morpholin-4-ylmethyl]-pyrrolidine-1-carboxylic acid tert-butyl ester as a white solid (915 mg). 1H-NMR (300 MHz, DMSO-d6): δ 11.30 (s, 1); 9.88 (s, 1); 9.04 (s, 1); 8.39-8.37 (m, 1); 8.20-8.15 (m, 2); 7.95 (s, 1); 3.99-3.82 (m, 3); 3.69-3.63 (m, 2); 3.44-3.32 (m, 1); 3.27-3.11 (m, 3); 2.92-2.80 (m, 1); 2.44-2.32 (m, 1); 1.99-1.67 (m, 5); 1.33 (s, 9). HCOOH standard conditions. DAD Rf=1.39 min. M+H=514. Chiral HPLC.

1. concentrationconcentrated to a residue
2. concentrationThe residue was concentrated once more from CH2Cl2
3. customto yield a yellow-brown solid which
4. temperatureGentle warming
5. dissolutiondissolution
6. stirringThe solution was stirred at RT for 30 min
7. customquenched via addition of 1N aqueous NaOH (30 ml)
8. additionThe mixture was poured into a separatory funnel
9. additioncontaining EtOAc (100 ml), H2O (100 ml), and brine (100 ml)
10. stirringThe mixture was shaken
11. customthe layers were separated
12. extractionThe aqueous layer was extracted with EtOAc (2×50 ml)
13. washthe combined organic layers were washed with brine
14. customThe organic layer was dried
15. filtrationfiltered
16. concentrationconcentrated
17. customto yield a light brown solid