Ammonia methanol → HCID 178476362, compound

Participants

Reaction Steps

To a mixture of 184 mg of 4-cyano-1-(2,3,5-tri-O-acetyl-β-D-ribofuranosyl)imidazolium-5-olate and 1 ml of dry methanol was added 15 ml of 15% (W/W) ammonia/methanol solution under cooling, and the mixture was stirred for two hours at the same temperature. Evaporation of the reaction mixture in vacuo gave a residue, which was purified by column chromatography on reversed phase silica gel [Merck RP-8 eluted with aqueous 10% methanol containing acetic acid (1%)] to give 100 mg of 4-cyano-1-(5-O-acetyl-β-D-ribofuranosyl)imidazolium-5-olate and 32 mg of 4-cyano-1-β-D-ribofuranosylimidazolium-5-olate, which was identified with the authentic sample of Example 2.

Provenance & Evidence