Fumaric Acid → L-Malic Acid

HRID 569726Notes

Reaction equation

[C:1]([OH:8])(=[O:7])/[CH:2]=[CH:3]/[C:4]([OH:6])=[O:5].C([O-])(=O)/C=C/C([O-])=[O:13]>>[C:1]([OH:8])(=[O:7])[C@H:2]([CH2:3][C:4]([OH:6])=[O:5])[OH:13]

Participants

Reactant 2

HCID 444972Fumaric Acid

HCID 5460307Fumarate

Product 1

HCID 222656L-Malic Acid

Procedure

An NH4 fumarate suspension with 400 g/l (calculated as fumaric acid) is introduced in a vessel and set at pH 8.0. The fumarate is first introduced partly in undissolved form. 35 g/l of cell mass (calculated as dry mass)--grown according to example A--is suspended in this liquid. After 36 hours incubation at 32° C., 378 g of L-malic acid per liter of fermentation liquid is produced.

Workup

custom35 g/l of cell mass (calculated as dry mass)

Sources & evidence

ORD record: ord-577890593cf44080921b8c0589328d60
Source dataset: uspto-grants-1988_03
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04734368

Reaction notes

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