反应详情

A solution of 3-{(1S)-1-[(1,1-dimethylethyl)oxy]-2-hydroxyethyl}-4-(3,4-dimethylphenyl)-2-methyl-1(2H)-isoquinolinone (4 mg, 10.54 μmol) in DMSO is treated with DMP (13 mg, 0.032 mmol). After 18 h, the reaction mixture was filtered through a pad of silica gel and washed with 1:1 EtOAc-hexanes. The filtrate was concentrated, dissolved in EtOAc and washed with H2O (3×), brine, dried (Na2SO4), filtered and concentrated. The residue was then dissolved in tert-Butanol (51.5 μl) and Tetrahydrofuran (THF) (51 μl) and treated with 2,3-dimethyl-2-butene (3 μl, 0.02 mmol) and a solution of NaClO2 (1.787 mg, 0.016 mmol) and NaH2PO4 (1.2 mg, 10.5 μmol) in water (100 uL). The crude reaction mixture was diluted with water and extracted with EtOAc (3×). The combined organics were washed with brine, dried (Na2SO4), filtered and concentrated. The residue was dissolved in DMF (250 uL) and purified by Gilson to afford the title compound: Rt=0.95, 0.97 (atropisomers), ES+ MS: 394 (M+1). The chirality of the above compound was established by comparison to authentic material of (R)- and (S)-(tert-butoxy)-2-(4-(3,4-dimethylphenyl)-2-methyl-1-oxo-1,2-dihydroisoquinolin-3-yl)acetic acid using a Chiral SFC 20% MeOH/80% CO2; 4.6×250 mm IC column; Rt (R)=1 min; (S)=7.22 min.

1. filtrationthe reaction mixture was filtered through a pad of silica gel
2. washwashed with 1:1 EtOAc-hexanes
3. concentrationThe filtrate was concentrated
4. dissolutiondissolved in EtOAc
5. washwashed with H2O (3×), brine
6. dry with materialdried (Na2SO4)
7. filtrationfiltered
8. concentrationconcentrated
9. dissolutionThe residue was then dissolved in tert-Butanol (51.5 μl)
10. customThe crude reaction mixture
11. extractionextracted with EtOAc (3×)
12. washThe combined organics were washed with brine
13. dry with materialdried (Na2SO4)
14. filtrationfiltered
15. concentrationconcentrated
16. dissolutionThe residue was dissolved in DMF (250 uL)
17. custompurified by Gilson