HuagongsheHuagongshe Chemistry Print Report · For research reference only
Biotin-X-NHS → 4-{Hydroxy-[3-(3-{6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-4-yl)-pentanoylamino]-hexanoylamino}-propoxy)-phenyl]-phenyl-methyl}-benzoic acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Biotin-X-NHS | HCID 5063989 | — |
| Reactant | 4-{[3-(3-Amino-propoxy)-phenyl]-hydroxy-phenyl-methyl}-benzoic acid | HCID 59987959 | — |
| Solvent | Dimethylformamide | HCID 6228 | — |
| Solvent | Chloroform methanol | HCID 21584188 | — |
| Product | 4-{Hydroxy-[3-(3-{6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-4-yl)-pentanoylamino]-hexanoylamino}-propoxy)-phenyl]-phenyl-methyl}-benzoic acid | HCID 72615106 | — |
Reaction Steps
A mixture of Trityl amino acid (4, 100 mg, 0.26 mM) and Biotin-X-NHS (113 mg, 0.25 mM) was stirred at room temperature in 3 mL of anhydrous DMF for overnight. After that DMF was removed under high vacuum and the residue obtained was passed through silica gel column using 50% CH3OH/CHCl3 as a solvent. Evaporation of the solvent yielded biotinylated trityl acid 5. (97.8%). Mass: 739 (M Na+), 715 (M-H).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US09034798B2 |
| Source dataset | uspto-grants-2015_05 |
| ORD ID | ord-e9fe858c7db44546a6e712365513cc1f |
| Source note | uspto-grants-2015_05 |