1,2-Dichloroethane → HCID 88569887, compound

Participants

Reaction Steps

A mixture of 31 mg (47 μmol) of the compound of Example 21, 13 mg (70 μmol) of EDC and 9.4 mg (70 μmol) of HOBt in 2 ml of DMF was stirred at RT for 20 min and then poured into 2 ml of ammonia (35% strength solution in water). The reaction mixture was stirred at RT for 30 min, then briefly concentrated on a rotary evaporator, acidified with 1N hydrochloric acid and separated by preparative HPLC [Method 6]. The appropriate fraction was concentrated on a rotary evaporator and the residue was dried under high vacuum. This gave 29 mg (92% of theory) of the title compound.

Provenance & Evidence