反应详情

Sodium borohydride (25 mg, 0.66 mmol) was added portionwise to a solution of tert-butyl 4-[6-[6-(6-formylpyrazin-2-yl)pyrazolo[4,3-c]pyridin-1-yl]-2-pyridyl]piperazine-1-carboxylate (162 mg, 0.33 mmol) in 6 ml of methanol. The mixture was stirred for 1 hour, concentrated in vacuum, the residue partitioned between ethyl acetate and water. The organic extracts were washed with water, 1% aq. citric acid, water, and brine, dried over sodium sulfate and concentrated. The residue was purified on a 12 g silica gel column eluting with 0-7% gradient of methanol in DCM to afford 125 mg (77%). MS (ESI) m/z: 489.2 [M+H]+

1. concentrationconcentrated in vacuum
2. customthe residue partitioned between ethyl acetate and water
3. washThe organic extracts were washed with water, 1% aq. citric acid, water, and brine
4. dry with materialdried over sodium sulfate
5. concentrationconcentrated
6. customThe residue was purified on a 12 g silica gel column
7. washeluting with 0-7% gradient of methanol in DCM
8. customto afford 125 mg (77%)