Glutaric anhydride → HCID 178455886, compound

Participants

Reaction Steps

3.0 mg (3.3 μmol) of α-amanitin, dried in vacuo over P4O10 was dissolved in 0.25 ml of dry pyridine and reacted with 0.9 mg (79 μmol) glutaric anhydride in 0.1 ml pyridine for 24 h at RT in the dark. The peptide was precipitated by addition of 7 ml of dry diethylether, centrifuged, and the solid washed a second time with diethylether and centrifuged. By way of this reaction an α-amanitin derivative is obtained wherein R1=—OH (in FIG. 1) is replaced by R1=—O—C(O)—(CH2)3—COOH.

Provenance & Evidence