Water → HCID 68296003, compound

Participants

Reaction Steps

4-(4-{Bis-[2-(tetrahydro-pyran-2-yloxy)-ethyl]-sulfamoyl}-4,7-diaza-spiro[2.5]oct-7-yl)-pyrrolo[2,3-d]pyrimidine-7-carboxylic acid tert-butyl ester (intermediate 17) was treated with a mixture of TFA (0.5 mL), H2O (0.2 mL) and THF (0.2 mL) at 40° C. for 1 h. The crude reaction mixture was neutralised using sat. NaHCO3 and then extracted with EtOAc (3×10 mL). The combined organic phases were dried (Na2SO4), filtered and concentrated in vacuo. The pure compound was obtained by standard preparative HPLC purification of the reaction mixture.

Provenance & Evidence