Water → ((3S)-6-[(2,4-Difluorobenzyl)oxy]-2,3-dihydro-1-benzofuran-3-yl)acetic acid

Temperature

55 °C

To a stirred mixture of methyl(3S)-(2,3-dihydro-6-hydroxy-1-benzofuran-3-yl)acetate (20.8 mg) and K2CO3 (20.7 mg) in DMF (0.5 mL) was added 2,4-difluorobenzyl chloride (24.4 mg) at room temperature. After stirring at 55° C. for 15 h, water (2.0 mL) and EtOAc (3.0 mL) were added to the mixture with stirring. The organic layer was separated by phase separation filter kit, and the filtrate was evaporated by blowing away with the air at 60° C. The resulting residue was dissolved in MeOH (0.5 mL) and 1N LiOH (0.3 mL), and the mixture was stirred at room temperature for 1 h. To the mixture was added 1N HCl (0.2 mL) and the solution was evaporated by blowing away with the air at 60° C. The resulting residue was dissolved in DMSO (1.0 mL) and the solution was purified by preparative HPLC (C18, eluent: water/CH3CN (including 5 mM AcONH4)). The eluted fraction was evaporated by air blowing at 60° C. to give the title compound (11.0 mg, 34.4%).

1. stirringwith stirring
2. customThe organic layer was separated by phase separation
3. filtrationfilter kit
4. customthe filtrate was evaporated
5. customby blowing away with the air at 60° C
6. dissolutionThe resulting residue was dissolved in MeOH (0.5 mL)
7. stirring1N LiOH (0.3 mL), and the mixture was stirred at room temperature for 1 h
8. additionTo the mixture was added 1N HCl (0.2 mL)
9. customthe solution was evaporated
10. customby blowing away with the air at 60° C
11. dissolutionThe resulting residue was dissolved in DMSO (1.0 mL)
12. customthe solution was purified by preparative HPLC (C18, eluent: water/CH3CN (including 5 mM AcONH4))
13. customThe eluted fraction was evaporated by air
14. customblowing at 60° C.

ORD record

ord-0a04ae40300e428f812bf3081d67fec1

Source dataset

uspto-grants-2016_01

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US09238639B2