HCID 50999194, compound → HCID 178513964, compound

Participants

Reaction Conditions

Reaction Steps

In a similar fashion to Compound LXXXVII, (1S,2S,3R,4R)-3-(2,3-Diamino-5-bromo-pyridin-4-ylamino)-bicyclo[2.2.1]hept-5-ene-2-carboxylic acid amide (50 mg, 0.148 mmol) and N-tert-butoxycarbonyl-6-carboxy-3,4-dihydrospiro[2H-1-benzopyran-2,4′-piperidine] (75.4 mg, 0.228 mmol) were reacted. The crude reaction was concentrated, then taken up into 4N HCl/dioxane and heated at 50° C. for 4 hours. The reaction mixture was then concentrated and chromatographed on Gilson HPLC 0-45% CH3CN/H2O. The desired fractions were lyophilized to produce 65 mg (47%) of the title compound as the TFA salt. mp: lyophilate, 1H NMR (300 MHz, DMSO-d6): 13.19 (s, 1H), 8.59 (m, 1H), 8.42 (m, 1H), 8.03 (s, 1H), 7.92 (s, 1H), 7.89 (d, 1H), 7.76 (s, 1H), 7.25 (s, 2H), 7.00 (d, J=8 Hz, 1H), 6.38 (s, 2H), 5.19 (t, J=8 Hz, 1H), 3.23 (m, 2H), 3.14 (m, 2H), 2.88 (m, 3H), 2.78 (s, 1H), 2.62 (d, J=8 Hz, 1H), 2.24 (d, J=8 Hz, 1H), 1.96-1.85 (m, 4H), 1.39 (d, J=8 Hz, 1H). MS: 551 (M+H), HPLC: 1.94 min (G method).

Provenance & Evidence