反应详情

TFA (0.50 mL, 6.5 mmol) was added to tert-butyl 4-(5-((4-(2-(1-amino-1-oxopropan-2-yl)phenethyl)-5-(trifluoromethyl)pyrimidin-2-yl)amino)pyridin-2-yl)piperidine-1-carboxylate A72 (0.060 g, 0.099 mmol) in DCM (5 mL) and the mixture was stirred for 2 hours. The mixture was concentrated under reduced pressure and quenched with 25% aq. NaOH (20 mL). The aqueous phase was extracted with EtOAc (3×20 mL) before the combined organics were dried (phase separation cartridge) and concentrated under reduced pressure. The suspension was sonicated with hexane and concentrated under reduced pressure to give the title compound (0.066 g, 134% residual solvent/water). LCMS-D: rt 3.025 min; m/z 499.3 [M+H]+.

1. concentrationThe mixture was concentrated under reduced pressure
2. customquenched with 25% aq. NaOH (20 mL)
3. extractionThe aqueous phase was extracted with EtOAc (3×20 mL) before the combined
4. customorganics were dried
5. custom(phase separation cartridge)
6. concentrationconcentrated under reduced pressure
7. customThe suspension was sonicated with hexane
8. concentrationconcentrated under reduced pressure