反应详情

温度

60 °C

Ammonia (7.0 M in methanol, 1.5 mL, 10.5 mmol) was added to a flask containing methyl (2S)-3-[4-(3-{[4-(difluoromethyl)pyrimidin-2-yl]amino}-5-methylphenyl)-1H-pyrazol-1-yl]-2-hydroxypropanoate (57.2 mg, 0.14 mmol) and the mixture was stirred overnight at 60° C. The mixture was cooled to room temperature and concentrated under reduced pressure. The residue was purified by reverse phase HPLC (0 to 95% ACN/water with 0.1% TFA modifier) to afford (2S)-3-[4-(3-amino-5-methylphenyl)-1H-pyrazol-1-yl]-2-hydroxypropanamide. MS ESI calcd. for C13H17N4O2 [M+H]+ 261. found 261.

1. temperatureThe mixture was cooled to room temperature
2. concentrationconcentrated under reduced pressure
3. customThe residue was purified by reverse phase HPLC (0 to 95% ACN/water with 0.1% TFA modifier)