Acetic Acid → HCID 131710691, compound

Participants

Reaction Steps

3-Acetoxymethyl-7β-formamidoceph-2-em-4α-carboxylic acid (1.5 g, 0.5 mmol) was dissolved in dioxan (12 ml) containing 50% conc. hydrochloric acid (1.7 ml.) at room temperature. After 2 hr. the excess solvent was removed by freeze-drying to give solid hydrochloride (1.5 g., 100%). Adjustment of the pH to the isoelectric point with a dilute ammonia solution failed to give a precipitate of the zwitterion. The infrared and n.m.r. results on the freeze-dried solution were consistant with the structure of the compound named in the title. Paper electrophoresis was carried out on Whatman No. 1 paper at pH 1.9 [98% formic acid (16.7 ml), glacial acetic acid (84 ml.), acetone (105 ml) and water (495 ml)] at a potential drop of 125 volts/cm for 20 min. The mobility obtained was 5 cm as compared with 3-acetoxymethyl-7β-aminoceph-3-em-4-carboxylic acid which has a mobility of 3.5 cm.

Provenance & Evidence