Arnebin I → Shikonin

Participants

Reaction Steps

Weigh 5.0 mg of shikonin, β,β-dimethylacrylshikonin, acetylshikonin obtained in Preparation example 1 or Preparation example 2, respectively. Dissolve the compounds in 1 ml DMSO. After diluting by 50 times with RPMI-1640 culture medium, pack them separately and further dilute into the following concentration: 100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78, 0.39 (μg/ml).

Provenance & Evidence