反应详情

67 mg (0.42 mmol) 1,2,3,4,5,6-hexahydro-[4,4′]bipyridinyl were added at RT to a solution of 182 mg (0.38 mmol) (R)-2-(4-hydroxy-3,5-dimethyl-phenyl)-1-carboxy-ethyl 4-(2-oxo-1,2,4,5-tetrahydro-1,3-benzodiazepin-3-yl)-piperidine-1-carboxylate, 133 mg (0.42 mmol) TBTU and 66 μL (0.47 mmol) triethylamine in 10 mL THF and 2 mL DMF and the reaction mixture was stirred for 2 h. 20 mL EtOAc were added, the organic phase was washed with 20 mL semisaturated NaHCO3 solution and dried on Na2SO4. After the elimination of the desiccant and solvent the residue was purified by HPLC. The fractions containing the product were combined, evaporated down, the residue was made alkaline with semisaturated NaHCO3 solution, extracted with 50 mL EtOAc and the organic phase was dried on Na2SO4. After the elimination of the desiccant and solvent the residue was stirred with DIPE, suction filtered and dried.

1. washthe organic phase was washed with 20 mL
2. customdried on Na2SO4
3. customAfter the elimination of the desiccant and solvent the residue was purified by HPLC
4. additionThe fractions containing the product
5. customevaporated down
6. extractionextracted with 50 mL EtOAc
7. customthe organic phase was dried on Na2SO4
8. stirringAfter the elimination of the desiccant and solvent the residue was stirred with DIPE, suction
9. filtrationfiltered
10. customdried